Journal: PLoS ONE

Article Title: Candidate microRNA Biomarkers in Human Gastric Cancer: A Systematic Review and Validation Study

doi: 10.1371/journal.pone.0073683

Figure Lengend Snippet: The characterestics of the eight microRNA profiling studies included in this systemic review.

Article Snippet: Oh et al , 2011 , Agilent Human miRNA microarray(V2) , 80 , 80 , 40 , 40.

Techniques: Microarray

Journal: International Journal of Cancer

Article Title: miR ‐17‐3p promotes the proliferation of multiple myeloma cells by downregulating P21 expression through LMLN inhibition

doi: 10.1002/ijc.33528

Figure Lengend Snippet: Expression of miR‐17‐3p in the bone marrow of multiple myeloma (MM) patients. A, miRNA 4.0 microarray showed that miR‐17‐3p is more highly expressed in the bone marrow of newly diagnosed MM (NDMM) patients than in that of healthy subjects. Number 1 on the orange background represents the NDMM patient and the number 2 on the green background represents the healthy subjects. B, Expression of the first nine upregulated miRNAs and two downregulated miRNAs in NDMM patients from the bone marrow mononuclear cells from four patients and four healthy samples. C‐F, Expression of miR‐17‐3p in the bone marrow of healthy subjects and NDMM patients (C), RRMM patients (D), CRMM patients (E), and MGUS patients (F). G, Expression of miR‐17‐3p in the bone marrow of NDMM and RRMM patients. H, RT‐PCR analysis of miR‐17‐3p expression in Grade I (n = 39), Grade II (n = 31) and Grade III (n = 22) MM patients. U6 was the internal reference. The results were analyzed by the 2 (−△△CT) method (* P < .05; ** P < .01; *** P < .001) [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: The miRNA profile was determined and analyzed by GeneChip miRNA 4.0 Array (Affymetrix, USA) from the Affymetrix Microarray system (Affymetrix, USA).

Techniques: Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction

Journal: Oncotarget

Article Title: Gain-of-function miRNA signature by mutant p53 associates with poor cancer outcome

doi: 10.18632/oncotarget.7090

Figure Lengend Snippet: ( A ) Schematic representation of the study procedures. Mutant p53 or empty vector was stably transfected to p53-null H1290 cancer cells, followed by Affymetrix miRNA 4.0 microarray study and differential expression analysis. ( B ) Venn's diagram showing the common and unique miRNAs regulated by either mutp53 or wtp53. ( C) The Circos map indicates the miRNAs that were upregulated (red) or downregulated (blue) by mutp53 R282W. The chromosomal locations of regulated miRNAs are also indicated.

Article Snippet: After total RNA isolation, microarray assay (Affymetrix miRNA 4.0) was used to measure the expression profile of miRNAs.

Techniques: Mutagenesis, Plasmid Preparation, Stable Transfection, Transfection, Microarray, Expressing